primary antibodies of rabbit anti-cd14  (Servicebio Inc)

Journal: Journal of Pain Research

Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

doi: 10.2147/JPR.S135939

Figure Lengend Snippet: Sequences of the primers used in this study

Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

Techniques: Sequencing

Journal: Journal of Pain Research

Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

doi: 10.2147/JPR.S135939

Figure Lengend Snippet: CGRP immunolocalization and mRNA expression in synovial tissue. Notes: Synovial tissue stained with ( A ) DAPI (nuclei), ( B ) CD14 or ( C ) CGRP. ( D ) Merged image. ( E ) High-magnification merged image of ( D ). Scale bars =100 μm. ( F ) Real-time PCR analysis of CGRP expression in non-cultured CD14− and CD14+ fractions. All data are presented as mean ± standard error (n=5). * p <0.05. Abbreviations: CGRP, calcitonin gene-related peptide; DAPI, 4′,6-diamidino-2-phenylindole; PCR, polymerase chain reaction.

Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

Techniques: Expressing, Staining, Real-time Polymerase Chain Reaction, Cell Culture, Polymerase Chain Reaction

Journal: Journal of Pain Research

Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

doi: 10.2147/JPR.S135939

Figure Lengend Snippet: Effects of PGE2 on CGRP expression in cultured synovial fibroblasts and macrophages. Notes: Synovial fibroblasts and macrophages were stimulated in vitro with PGE2 (10 μM) or without PGE2 (vehicle) for 24 h prior to the extraction of total RNA for ( A ) CD14 and ( B ) CGRP expression analysis. All data are presented as mean ± standard error (n=5). * p <0.05. Abbreviations: PGE2, prostaglandin E2; CGRP, calcitonin gene-related peptide.

Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

Techniques: Expressing, Cell Culture, In Vitro, Extraction

Journal: PLoS ONE

Article Title: Fibroblast Growth Factor-9 Enhances M2 Macrophage Differentiation and Attenuates Adverse Cardiac Remodeling in the Infarcted Diabetic Heart

doi: 10.1371/journal.pone.0120739

Figure Lengend Snippet: A-O : Representative photomicrographs illustrate CD14 positive monocytes in red ( A , F , and K ), cardiac myocytes in green ( B , G , and L ), total nuclei in blue ( C , H , and M ), merged images ( D , I , and N ), and enhanced merged images ( E , J , and O ) for all control and experimental groups. White arrows are used to show the areas enhanced in D , I , and N . Scale bar = 75μm. P : Histogram of quantitative monocyte infiltration data 2 weeks post-MI. n = 5 animals/group. *p<0.05 vs. sham and #p<0.05 vs. MI.

Article Snippet: Slides were incubated with primary anti-rabbit antibodies either against CD14 (monocytes, Abbiotec, #25156, 1:50), iNOS (M1 macrophages, Abcam, #ab129372, 1:50), or CD206 (M2 macrophages, Abcam, #ab64693, 1:50) overnight at 4°C.

Techniques: Control

Journal: World Journal of Gastroenterology

Article Title: Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia

doi: 10.3748/wjg.v8.i3.551

Figure Lengend Snippet: Effect of CD14 Ab on production of TNF-α in supernatants of LSECs. bP < 0.01 vs CD14 Ab

Article Snippet: We stained LSECs with rabbit anti-rats primary antibody against CD14 from Santa Cruz Biotechnology and found this Ab against CD14 was effective for detecting the expression of CD14 protein.

Techniques:

Journal: World Journal of Gastroenterology

Article Title: Expression of CD14 protein and its gene in liver sinusoidal endothelial cells during endotoxemia

doi: 10.3748/wjg.v8.i3.551

Figure Lengend Snippet: Effect of CD14 Ab on production IL-6 in supernatants of LSECs. bP < 0.01 vs CD14 Ab

Article Snippet: We stained LSECs with rabbit anti-rats primary antibody against CD14 from Santa Cruz Biotechnology and found this Ab against CD14 was effective for detecting the expression of CD14 protein.

Techniques: