Journal: PLoS ONE
Article Title: Fibroblast Growth Factor-9 Enhances M2 Macrophage Differentiation and Attenuates Adverse Cardiac Remodeling in the Infarcted Diabetic Heart
doi: 10.1371/journal.pone.0120739
Figure Lengend Snippet: A-O : Representative photomicrographs illustrate CD14 positive monocytes in red ( A , F , and K ), cardiac myocytes in green ( B , G , and L ), total nuclei in blue ( C , H , and M ), merged images ( D , I , and N ), and enhanced merged images ( E , J , and O ) for all control and experimental groups. White arrows are used to show the areas enhanced in D , I , and N . Scale bar = 75μm. P : Histogram of quantitative monocyte infiltration data 2 weeks post-MI. n = 5 animals/group. *p<0.05 vs. sham and #p<0.05 vs. MI.
Article Snippet: Slides were incubated with primary anti-rabbit antibodies either against CD14 (monocytes, Abbiotec, #25156, 1:50), iNOS (M1 macrophages, Abcam, #ab129372, 1:50), or CD206 (M2 macrophages, Abcam, #ab64693, 1:50) overnight at 4°C.
Techniques: Control